HTEC-500/510

A sine wave over several minutes on the chromatogram is found during monitoring the baseline of the ECD. HTEC-500
A sine wave on the chromatogram is typically caused by fluctuations in temperature. Please ensure the HTEC door is closed securely. If the system i...
Fri, 4 Oct, 2019 at 2:01 PM
Auto Zero: Confirming the connection between AS-700 and HTEC-500/510
Please ensure the orange power strip is fully pushed in. Check for loose cables. Please touch the autozero terminals with forceps to short them on ...
Fri, 4 Oct, 2019 at 2:02 PM
Baseline wanders, drifting.
A wandering baseline in a large range slowly over an hour might related to pressure change such as small leak from the precolumn or another source.  Other p...
Fri, 4 Oct, 2019 at 2:02 PM
Broad peaks with a manual injection
If you notice peaks are much broader when using the manual injector vs. the autosampler, please ensure the autosampler’s valve is set to the LOAD position. ...
Fri, 4 Oct, 2019 at 2:02 PM
Clogged Degasser
If the system has not been operated in a few weeks or longer, the degasser channel may be clogged by crystallization caused as the mobile phase present in t...
Fri, 4 Oct, 2019 at 2:03 PM
ECD Background, What is it? What is the standard read?
When the columns are set up and mobile phase is allowed to flow at a certain condition before injecting samples, the ECD monitors the current, which is ca...
Wed, 30 Sep, 2020 at 5:04 PM
ECD baseline blip, shift, unknown peak.
(See image blelow) This is related to air bubble generation in the detector cell. Please do not put a waste container below the HPLC system; put it on the ...
Tue, 16 Jul, 2019 at 3:37 PM
How to Clean the Check Valves
1. Remove the check valve assembly from the pump head. 2. Put some acetone in a small beaker. Over the beaker of acetone, fasten a column end plug to the a...
Mon, 30 Sep, 2019 at 1:46 PM
Where does epinephrine elute on CAX column?
 Under standard separation conditions, Epi has a retention time of 5.9mins. The peak is not well resolved from norepinephrine which elutes at 5.4mins. 
Thu, 18 Jul, 2019 at 10:06 AM
HTEC Pump Errors
Below are display errors for the HTEC-500/510, possible causes, and solution. Error 1-Upper Pressure Limit Error Cause: Pressure is higher than the ...
Fri, 11 Dec, 2020 at 8:17 AM